Immunology tests

Diagnostic Immunology and Flow Cytometry Tests Available and Specimen Requirements

Test

CODE

Sample

Volume

Special Requirements

†ABPA see IgE specific for aspergillus

IgESP

Alpha-1-Antitrypsin (AAT)

AAT

serum (red)

3.0ml

NA

ANA see anti-nuclear

ANAI

†ANCA see anti-neutrophil cytoplasmic

ANCA

†Anti-Cardiolipin Ab (MACL)

APL

serum (red)

3.0ml

NA

†Anti-Diptheria toxin Ab (Diptheria)

DIP

serum (red)

3.0ml

NA

Anti-DNA/EIA

DNA

serum (red)

3.0ml

NA

†Anti-DNP

serum (red)

3.0ml

NA

Anti-endomysial Ab (panel only) Now Anti-tTG

CDAP

serum (red)

3.0ml

NA

Anti-Gliadin (Panel only)

CDAP

serum (red)

3.0ml

NA

†Anti-Hemophilus Influenza B

HIB

serum (red)

3.0ml

NA

†Anti-HIB see anti-hemophilus

HIB

Anti-mitochondria Ab

AMCA

serum (red)

3.0ml

NA

†Anti-neutrophil cytoplasmic Ab

ANCA

serum (red)

3.0ml

NA

Anti-nuclear antibody (ANA)

ANAI

serum (red)

3.0ml

NA

†Anti-phosphatidyl serine see anti-cardiolipin

APL

†Anti-phospholipid see anti-cardiolipin

APL

†Anti-PM1 and JO

PMJO

serum (red)

3.0ml

NA

†Anti-pneumococcal Ab

PNU

serum (red)

3.0ml

NA

Anti-ribonucleoprotein (RNP)

RNP

serum (red)

3.0ml

NA

Anti-SCL70

ASCL

serum (red)

3.0ml

NA

Anti-Smith (Anti-Sm)

ASM

serum (red)

3.0ml

NA

Anti-smooth muscle

ASMA

serum (red)

3.0ml

NA

Anti-SSA/Ro

ASSA

serum (red)

3.0ml

NA

Anti-SSB/La

ASSB

serum (red)

3.0ml

NA

Anti-tTG (see anti-endomysial)

Anti-tetanus

ATET

serum (red)

3.0ml

NA

Antigen Stimulation Panel

Heparin

All 10 ml

requires scheduling

†Antigliadin Ab

CDAP

serum (red)

3.0ml

NA

Bb Alternative Complement Fragment†

Plasma

EDTA

3.0

collect on ice or freeze as 2 aliquots

B27 see HLA-B27

C-Reactive Protein

CRP

serum (red)

3 ml

†C1 esterase inhibitor

C1ES

serum (red)

3.0ml

NA

†C1q(immune complex)

C1Q

serum (red)

3.0ml

collected on ice or freeze as 2 aliquots

C3

C3

serum (red)

3.0ml

NA

C3a Complement Activation product†

C3a

Plasma Futhan EDTA

3.0 ml

Collected on ice or freeze as 2 aliquots

C4

C4

serum (red)

3.0ml

NA

C4d/C4† Complement activation product (C4d/C4)

C4ACT

Plasma futhan EDTA

5.0ml

collected on ice or freeze as 3 aliquots Must be pre-scheduled

Complement Activation† products, C3a, C4/C4d/C5a

see

indiv.

test

Plasma futhan EDTA

2 frozen aliquots per test

†C4 Allotyping

C4AT

serum (red)

4 ml

keep on ice or freeze as 2-1 ml aliquots immediately

C4a Complement Activation product†

Plasma futhan EDTA

2 frozen aliquots per test

C5a Complement Activation

product†

C5a

Plasma Futhan EDTA

3.0 ml

Collected on ice or freeze as 2 aliquots

CD40-Ligand

CD40L

1-Heparin and 1-EDTA

3.0 ml of each

keep at room temperature & please schedule in advance

†C5b-9

TCCS

plasma EDTA

3.0ml

collect on ice or freeze as 2 aliquots

CGDF- Chronic Granulomatous Disease by Flow (used to be NBT)

CGDF

Green top

3 ml

keep at room temp. and transport immed. please schedule in advance

CH50 see Total hemolytic

THC

†Complement C2

MC2

serum (red)

3.0ml

collect on ice or freeze

Crithidia (anti nDNA)

CRITH

serum (red)

3.0ml

NA

Cryoglobulins

CRYO

serum (red)

3.0ml

collected into warm tube and kept warm (37°C)

HLA B27

B27

heparin (green)

10ml

keep at room temperature do not order on Fri past 11 am or on Saturdays

†HLA typing phone prior to draw

HLA

Hyper IgM (CD40L) assay

CD40L

Green top

and

purple top

3ml

+

3ml

keep at room temp. and transport immed. please schedule in advance

iC3b† Complement activation

Plasma EDTA

3.0 ml

Collected on ice or freeze as 2 aliquots

IgA

IgA

serum (red)

3.0ml

NA

IgE

IgE

serum (red)

3.0ml

NA

†IgE specific for Aspergillus, (ABPA)

IgESP

serum (red)

3.0ml

Requires Patient history documentation before test will be performed

IgG

IgG

serum (red)

3.0ml

NA

IgG subclasses

IgS

serum (red)

3ml

NA

IgM

IgM

serum (red)

3.0ml

NA

Immune complex Panel (C1q + Raji)

IMC

serum (red)

3.0ml

collect on ice or freeze as 3 aliquots

Immunoelectrophoresis (IEP)

Not Avail.

3.0ml

See immunofixation

Immunofixation Electrophoresis (IFE)

IEP

serum (red)

3.0ml

NA

LAD Leukocyte adhesion Deficiency Type-1

LAD

EDTA (purple)

3 ml

keep at room temp. and transport immed. please schedule in advance

†Lyme testing

LYM

serum (red)

3.0ml

NA

Lymphocyte Phenotyping

Flow cytometry studies

FCSL

plasma EDTA (purple)

3-5ml

Room temperature-CBC and Diff. must be ordered

Microsomal Ab only (see Thyroid Microsomal)

ABM

serum (red)

3 ml

Also called Thyroid Peroxidase

Mitogen Stimulation

MSP

Heparin

Full 10ml

requires scheduling

Myeloperoxidase† (MPO)

Misc

Purple top

3 ml

Sent out No Fridays

NBT (see Nitroblue)

Neopterin

NEOP

serum (red)

3.0ml

Freeze 1 -0.5 ml aliquot

Nitroblue Tetrazoleum Dye Reduction test (NBT) See CGDF

Now performed by Flow Cyt.

Heparin (green)

Full 10ml

requires scheduling and keep at room temperature

Peroxidase (Thyroid) see Thyroid Microsomal

†Pneumo titre see Anti-pneumococcal

PNU

RAJI (immune complex)

RAJI

serum (red)

3.0ml

collect on ice or freeze as 2 aliquots

RAST-order by individual allergen code!

RAST

serum (red)

0.1 ml per aller.

Must indicate individual Allergens

Rheumatoid factor

RF

serum (red)

3.0ml

NA

Surface markers, see T and B cell immunophenotyping

FCSL

T and B cell immunophenotyping

FCSL

EDTA (purple)

4ml

Room temp. Do not refrigerate! Requires Diagnosis

THC see Total hemolytic Complement

THC

Thyoglobulin & Microsomal Ab

Anti-thyroid Ab panel

TAB

serum (red)

3 ml

Anti-thyroglobulin & Anti-peroxidase

Thyroglobulin Ab only

ABT

serum (red)

3 ml

Thyroid microsomal Ab only (see Thyroid Microsomal)

ABM

serum (red)

3 ml

Also called Thyroid Peroxidase

Thyroid Peroxidase (Same as Thyroid Microsomal)

Peroxidase-Thyroid

use Microsomal!

Total hemolytic complement (THC or CH50)

THC

serum (red)

5.0ml

collect on ice or freeze as 2 aliquots

† - these tests are sent out ie. not performed in the Diagnostic Immunology and Flow Cytometry Laboratory


Content last reviewed: May 2007